The length of the inserted fragments of the forward and backward subtracted cdna libraries was 360 and 160 base pairs in average respectively by pcr detection . the results showed that the subtracted libraries constructed were suitable for further study on the genes related to the ovary development of the mitten crab Pcr扩增鉴定正、反向差减cdna文库的插入片段平均大小分别为360bp和160bp ，表明所构建的差减文库适合进一步研究中华绒螯蟹卵巢发育相关基因。
